Investigation of the Antimicrobial, Antioxidant and Anti- Inflammatory Activity of Compound Isolated from Murraya Koenigii

In the present investigation, the compound responsible for antioxidant, antimicrobial and anti-inflammatory properties in methanolic extract of leaves of Murraya koenigii L. was determined by PerkinElmer GC Claurus 500 system and Gas Chromatograph interfaced to a Mass Spectrometer GC/MS technique. GC-MS analysis of methanol extract of the leaves of the plant revealed the existence of 1Methyl-pyrrolidine-2-carboxylic acid (69.00%), Ethyl α-d glucopyranoside (13.36%), Isolongifolene, Isolongifolene (3.68%), c-Himachalene (2.88%), 1,2-Ethanediol, monoacetate (2.79%), 1,2Benzenedicarboxylic acid, di-isooctyl ester (2.55%). The pure compounds were separated using a Shimadzo LC 2010 HPLC system (Kyoto, Japan), equipped with a Shimadzo LC 2010 UV-VIS detector with a thermostated flow cell and a selectable two wavelengths of 190 370 nm or 371–600nm. These were further screened for their antimicrobial, antioxidant and anti-inflammatory properties. All the compounds possessed some or the other activity. It was found that the compound 9, 12 octadecadienoic acid having the retention time 18.81 and the peak area 0.60 % had potent antioxidant, antimicrobial and anti-inflammatory properties. The compound showed potent antimicrobial activity against Bacillus subtilis, E.coli, Proteus vulgaris, Salmonella typhimurium, Staphylococcus aureus, Candida albicans, Saccharomyces cerevisae, Aspergillus niger and Penicillium notatum at MIC value from 0.05-0.56 μg/ml. The compound showed less activity against Pseudomonas aeruginosa in comparison to other pathogens. The compound possessed to have strong antioxidant activity with IC50 value of 45.65 μg/ml as measured by DPPH assay. The compound possessed 85 % reduction in paw edema at a dose of 150 μg/ml in reference to standard anti-inflammatory drug, aspirin which showed 68.62 % reduction. The compound was further assayed for cellular toxicity to fresh sheep erythrocytes and found to have no cellular toxicity.